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KMID : 0617819960060010198
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Journal of Wonkwang Oriental Medicine
1996 Volume.6 No. 1 p.198 ~ p.208
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Effects of Fructus Chebulae Extract on The Contraction of Isolated Rat Trachea Smooth Muscle
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Abstract
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Fructus Chebulae, a traditional herb medicine, has been used in Asia for many centuries as a treatment for diarrhoea, enteroffhagia, metrorrhagia, metritis and leukorrhoea. However, the effect of Fuructus Chebulae on tracheal smooth muscle is not known. The purpose of the present study was to determine the effect of Fuructus Chebulae on acetylcholine-, histamine-induced tracheal smooth muscle contraction in rats. Sprague Dawley rats(200g, female) were killed by CO_2 exposure and a segment (8-10mm) of the thoracic trachea from each rat was cut into equal segments and mounted ´in pairs´ in a tissue bath. Contractile force was measured with force displacement transducers under 0.5g loading tension. The dose of acetylcholine (Ach) which evoked 50% of maximal response (ED_50) were was obtained from cumulative dose response curves for acetycloline and histamine (10^-7 -10^-4M). Contractions evoked by Ach and His (ED_50) were inhibited significantly by Fructus Chebulae. The mean percent inhibition was 49±5% (p=0.05) after 30ul/㎖ Fuructus Chebulae, and 25±3% (p=0.01) after 100ul/㎖ Fructus Chebulae in Ach ED_50, and 53±6%(p=0.05) after 30ul/㎖ Fuructus Chebulae, and 30±4%(p=0.01) after 100ul/㎖ Fructus Chebulae in His ED_50. Propranolol (10^-7M) significantly attenuated the ingibitory effcts of Fuructus Chebulae. Following treatment with propranolol, the mean percent inhibition caused by 30ul/㎖ Fuctus Chebulae fell to 72±6% (p=0.01) and by 100ul/㎖ Fructus Chebulae fell to 55±4%(p=0.01) (n=7) in Ach and by 30ul/㎖ Fuctus Chebulae fell to 70±6% (p=0.01) and by 100ul/㎖ Fructus Chebulae fell to 57±4% (p=0.01) (n=7) in His. Propranolol(10^-7M) did not significantly after the inhibitory effect of Fructus Chebulae. These results indicate that Fructus Chebulae can relax acetylcholine-induced contraction of rat tracheal smooth muscle, and that this inhibition involves, in part, the relation to the symphatetic action.
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KEYWORD
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